They're transit marks identifying Manhattan post office stations through which the cover passed en route to its destination in present Chelsea. The address indicates General Theological Seminary at 20th St & Ninth Avenue; it's still there. To get there from Troy up the Hudson it had to transit several offices within Manhattan. It appears that the cover was moved from station F to C (or reverse) within the 10AM hour on 8 February. Dispatch occurred on 7 February so there was 1-day delivery from upstate NY to lower Manhattan at the time.
The lettered station IDs have been assigned to different locations over the years. This 1863 article identifies one set of designations.
http://www.nytimes.com/1863/09/30/n...-office.htmlAlso, technically the octagonal mark is a receiving or arrival back stamp, not a cancellation.
Chris